FTL antibody (AA 38-165) (Biotin)

Catalog No. ABIN6191655

Product Details anti-FTL Antibody (Biotin)

Target: FTL (Ferritin, Light Polypeptide (FTL))

Binding Specificity: AA 38-165

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTL antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: Mouse Monoclonal anti-Ferritin, Light chain (FTL/1388), Biotin Conjugate

Characteristics: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human FTL protein fragment (aa 38-165) (exact sequence is proprietary)

Clone: FTL-1388

Isotype: IgG2a

Application Details

Application Notes: Immunohistology (formalin) 0.1-0.2 μg/mL

• Flow cytometry 0.1-0.2 μg/million cells Immunofluorescence 0.1-0.2 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilution for a specific application should be determined by user

Comment:

HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for FTL

Target: FTL (Ferritin, Light Polypeptide (FTL))

Alternative Name: Ferritin, Light chain (FTL Products)

Synonyms: NBIA3 antibody, apoferritin antibody, ferritin antibody, fhc antibody, fth antibody, fth1 antibody, fthl6 antibody, ftn-2 antibody, pig15 antibody, plif antibody, FT antibody, Ftl antibody, Ftl-1 antibody, L-ferritin antibody, FTL antibody, Ftl1 antibody, FTL2 antibody, FTD antibody, ferritin light chain antibody, ferritin, heavy polypeptide 1 L homeolog antibody, ferritin light polypeptide 1 antibody, ferritin, light polypeptide antibody, ferritin light-chain antibody, ferritin light chain 1 antibody, FTL antibody, fth1.L antibody, Ftl1 antibody, CpipJ_CPIJ004069 antibody, Ftl antibody

Background: Ferritin L chain, Ferritin L subunit, Ferritin light chain, Ferritin light polypeptide, FTL, LFTD, NBIA3

Molecular Weight: 19-25 kDa

Gene ID: 2512, 433670

UniProt: P02792

Pathways: Transition Metal Ion Homeostasis