UHRF1 antibody (C-Term)

Catalog No. ABIN6243326

This Rabbit Polyclonal antibody specifically detects UHRF1 in WB. It exhibits reactivity toward Mouse.

Quick Overview for UHRF1 antibody (C-Term) (ABIN6243326)

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UHRF1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB51453

Product Details anti-UHRF1 Antibody

Binding Specificity: AA 596-628, C-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Mouse Uhrf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 596-628 amino acids from the C-terminal region of Mouse Uhrf1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for UHRF1

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Alternative Name: Uhrf1

Background: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.

Molecular Weight: 88304

UniProt: Q8VDF2