WEE1 antibody (Ser123)

Catalog No. ABIN6243968

This Rabbit Polyclonal antibody specifically detects WEE1 in WB. It exhibits reactivity toward Human.

Quick Overview for WEE1 antibody (Ser123) (ABIN6243968)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB57984

Product Details anti-WEE1 Antibody

Binding Specificity: AA 96-130, Ser123

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This Wee1(S123) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 96-130 amino acids from human Wee1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: Wee1

Background: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Molecular Weight: 71597

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase