ATF6 antibody (N-Term)

Catalog No. ABIN6251795

The Mouse Monoclonal anti-ATF6 antibody (Clone AF6229) (ABIN6251795) specifically detects ATF6 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ATF6 antibody (N-Term) (ABIN6251795)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: AF6229

Product Details anti-ATF6 Antibody

Binding Specificity: N-Term

Purification: Protein G purified monoclonal antibody

Immunogen: A recombinant protein fragment from the N-terminal region of human ATF6 was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Western blot: 2-4 μg/mL (2)

IHC (Paraffin): 5 μg/mL (1)

ATF6 is a 670 amino acid protein that migrates in western blot between 70~90 kD due to glycosylation.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. ATF6 is seen in western blotting between 75~90KD.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6

Background: ER stress can cause the accumulation of unfolded proteins and lead to the activation of the UPR (unfolded proetin response). Cells have developed an intracellular signaling pathway to deal with this stress, and Activating Transcription Factor 6 (ATF6) is a major transducer of the UPR. Upon activation, ATF6 seperates from GPR78 and is cleaved. The ~50 kD cytosolic portion of ATF6 translocates to the nucleus, transmitting the ER stress signal. In the nucleus, ATF6 activates the transcription of UPR-responsive genes by biniding to their ERSE (ER stress response element) region.

Gene ID: 22926

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response