TFF1 antibody (AA 57-84)
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- Target See all TFF1 Antibodies
- TFF1 (Trefoil Factor 1 (TFF1))
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Binding Specificity
- AA 57-84
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Reactivity
- Human, Cynomolgus
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TFF1 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Protein G affinity chromatography
- Immunogen
- Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the human protein were used as the immunogen for the Estrogen Inducible Protein pS2 antibody.
- Clone
- SPM313
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product TFF1 Primary Antibody
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- Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Optimal dilution of the Estrogen Inducible Protein pS2 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Target
- TFF1 (Trefoil Factor 1 (TFF1))
- Alternative Name
- Estrogen Inducible Protein pS2 (TFF1 Products)
- Synonyms
- BCEI antibody, D21S21 antibody, HP1.A antibody, HPS2 antibody, pNR-2 antibody, pS2 antibody, Bcei antibody, PS2 antibody, bcei antibody, d21s21 antibody, hp1.a antibody, hps2 antibody, p1 antibody, p1-a antibody, pnr-2 antibody, ps2 antibody, tff1 antibody, xP1 antibody, xp1-L antibody, xp1 antibody, TFF1 antibody, Ps2 antibody, trefoil factor 1 antibody, trefoil factor 1 S homeolog antibody, trefoil factor 1, gene 2 S homeolog antibody, TFF1 antibody, Tff1 antibody, tff1.S antibody, tff1.2.S antibody, LOC100358238 antibody
- Background
- It recognizes a polypeptide of 6.5 kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60 % of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
- Gene ID
- 7031
- UniProt
- P04155
- Pathways
- EGFR Signaling Pathway
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