THBD
Reactivity: Human
WB, FACS, IF
Host: Rabbit
Polyclonal
RB56919
unconjugated
Application Notes
Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml
IF: 0.5-1 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT
ELISA: 2-4 μg/mL, order the BSA-free formulation for coating
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.2 mg/mL
Buffer
PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot and Store at -20C. Avoid freez-thaw cycles.
It recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.