CD47 antibody

Catalog No. ABIN6251897

This anti-CD47 antibody is a Mouse Monoclonal antibody detecting CD47 in FACS, WB, IHC, IF and BI. Suitable for Human.

Quick Overview for CD47 antibody (ABIN6251897)

Target: CD47

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), BioImaging (BI)

Clone: B6H12-2

Product Details anti-CD47 Antibody

Purification: Protein G affinity chromatography

Immunogen: Intact CD47 purified from placenta was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Functional Activity (Blocking): Order BSA/Azide-free format

Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Western blot: 2-5 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50  kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Gene ID: 961

UniProt: Q08722