TOX3 antibody (AA 200-400)

Catalog No. ABIN6252144

This Mouse Monoclonal antibody specifically detects TOX3 in FACS, WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for TOX3 antibody (AA 200-400) (ABIN6252144)

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOX3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TOX3-1123

Product Details anti-TOX3 Antibody

Binding Specificity: AA 200-400

Purification: Protein G affinity chromatography

Immunogen: A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX3 antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the TOX3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0 or 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TOX3

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Alternative Name: TOX3

Background: It recognizes a 63  kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

Gene ID: 27324

UniProt: O15405

Pathways: Chromatin Binding