Endoglin antibody (AA 74-251)

Catalog No. ABIN6252250

The Mouse Monoclonal anti-Endoglin antibody is suitable to detect Endoglin in samples from Human. It has been validated for WB, FACS and IF.

Quick Overview for Endoglin antibody (AA 74-251) (ABIN6252250)

Target: Endoglin (ENG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endoglin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: ENG-1326

Product Details anti-Endoglin Antibody

Binding Specificity: AA 74-251

Purification: Protein G affinity chromatography

Immunogen: A partial recombinant protein corresponding to amino acids 74-251 of human CD105/Endoglin was used as the immunogen for the CD105 antibody.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 1-2 μg/mL

WB: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Titering of the CD105 antibody may be required for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105

Background: CD105/Endoglin is a Type I membrane glycoprotein located on cell surfaces and is part of the TGF-beta receptor complex. This protein has been found on endothelial cells, activated macrophages, fibroblasts, and smooth-muscle cells. CD105 has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. It is highly expressed in endothelial cells during tumor angiogenesis and inflammation, with weak or negative expression in vascular endothelium of normal tissues. Angiogenesis is a promising prognostic marker in a variety of tumors. Endoglin is a more specific and sensitive marker for tumor angiogenesis than CD31 or CD34, as it labels only newly-formed blood vessels and may serve as a prognostic marker for Prostate Adenocarcinoma, and cancers of the lung, stomach, breast, and brain.