BRCA1 antibody (AA 445-620)

Catalog No. ABIN6252367

The Mouse Monoclonal anti-BRCA1 antibody is suitable to detect BRCA1 in samples from Human. It has been validated for IF, IHC (p) and FACS.

Quick Overview for BRCA1 antibody (AA 445-620) (ABIN6252367)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: BRCA1-1398

Product Details anti-BRCA1 Antibody

Binding Specificity: AA 445-620

Purification: Protein G affinity chromatography

Immunogen: Amino acids 445-620 were used as the immunogen for the BRCA1 antibody.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 2-4 μg/million cells in 0.1ml

Immunofluorescence: 2-4 μg/mL

IHC (FFPE): 5-10 μg/mL

Optimal dilution of the BRCA1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: BRCA1 (Breast cancer type 1 susceptibility protein) is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene.

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus