TLR4 antibody

Catalog No. ABIN6252558

Product Details anti-TLR4 Antibody

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Rat, Dog, Monkey, Pig, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the TLR4 antibody.

Clone: TLR4-230

Isotype: IgG2a kappa

Application Details

Application Notes: Functional Activity (Order SAF formulation)

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Optimal dilution of the TLR4 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4 (TLR4 Products)

Synonyms: ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody

Background: This mAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins