Neurofilament antibody

Catalog No. ABIN6252790

The Mouse Monoclonal anti-Neurofilament antibody is suitable to detect Neurofilament in samples from Human, Rat, Mouse, Chicken and Pig. It has been validated for FACS, IHC and WB.

Quick Overview for Neurofilament antibody (ABIN6252790)

Target: Neurofilament

Reactivity: Human, Rat, Mouse, Chicken, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Neurofilament antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)

Clone: NF421

Product Details anti-Neurofilament Antibody

Purification: Protein G purified Neurofilament antibody

Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (2)

Prediluted format : incubate for 30 min at RT (3)

The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Neurofilament

Target: Neurofilament

Background: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.

Gene ID: 4744

UniProt: P12036