TIA1 antibody (AA 279-380)

Catalog No. ABIN6252793

The Mouse Monoclonal anti-TIA1 antibody is suitable to detect TIA1 in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for TIA1 antibody (AA 279-380) (ABIN6252793)

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TRIA1-1

Product Details anti-TIA1 Antibody

Binding Specificity: AA 279-380

Purification: Protein G affinity chromatography

Immunogen: Human partial recombinant protein (amino acids 279-380) was used as the immunogen for this TIA-1 antibody.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/10^6 cells

IF: 1-2 μg/mL

Western blot: 1-2 μg/mL

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIA-1 antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TIA1

Target: TIA1 (TIA1 Cytotoxic Granule-Associated RNA Binding Protein (TIA1))

Alternative Name: TIA-1

Background: T-Cell Intracellular Antigen-1 is a cytoplasmic granule-associated protein expressed in lymphocytes processing cytolytic potential. It is a member of an RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15  kDa protein thought to be derived from the carboxyl terminus of the 40  kDa product by proteolytic processing. TIA-1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.

Gene ID: 7072

UniProt: P31483