Retinoblastoma Protein (Rb) antibody

Catalog No. ABIN6252811

The Mouse Monoclonal anti-Retinoblastoma Protein (Rb) antibody is suitable to detect Retinoblastoma Protein (Rb) in samples from Human and Mouse. It has been validated for WB, IP and IHC (p).

Quick Overview for Retinoblastoma Protein (Rb) antibody (ABIN6252811)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Retinoblastoma Protein (Rb) antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB1-1754

Product Details anti-Retinoblastoma Protein (Rb) Antibody

Purification: Protein G affinity chromatography

Immunogen: A human recombinant protein was used as the immunogen for this Rb antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: WB: 0.5-1 μg/mL

IP: 1-2 μg/500 μg protein lysate

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Titering of the Rb antibody may be required for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Alternative Name: Retinoblastoma / Rb

Background: Recognizes a 105  kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Gene ID: 5925

UniProt: P06400

Pathways: Mitotic G1-G1/S Phases