Huntingtin antibody (AA 85-200)

Catalog No. ABIN6254174

There is 1 publication for this product available. The Goat Polyclonal anti-Huntingtin antibody is suitable to detect Huntingtin in samples from Human, Rat, Mouse, Rabbit, Monkey, Horse, Dog, Sheep, Pig, Hamster, Guinea Pig, Goat, Donkey, Cow, Chicken, Cat and Avian. It has been validated for WB, IF, IHC (p) and IHC (fro).

Quick Overview for Huntingtin antibody (AA 85-200) (ABIN6254174)

Target: Huntingtin (HTT)

Reactivity: Human, Rat, Mouse, Rabbit, Monkey, Horse, Dog, Sheep, Pig, Hamster, Guinea Pig, Goat, Donkey, Cow, Chicken, Cat, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This Huntingtin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Huntingtin Antibody

Binding Specificity: AA 85-200

Purpose: Anti-HTT

Sequence: PLHRPKKELS ATKKDRVNHC LTICENIVAQ SVRNSPEFQK LLGIAMELFL LCSDDAESDV RMVADECLNK VIKALMDSNL PRLQLELYKE IKKNGAPRSL RAALWRFAEL

Specificity: Reacts huntingtin protein, amino acids 85-200. The antibody recognizes wild type and mutant huntingtin.

Characteristics: Huntingtin

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 85 to 200 aa of human HTT produced in E. coli

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:500-1:2,000, IHC-P:1:500-1:2,000, IHC-F:1:500-1:2,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-Huntingtin antibody (ABIN6254174)

Meunier, Merienne, Jollé, Déglon, Pellerin: "Astrocytes are key but indirect contributors to the development of the symptomatology and pathophysiology of Huntington's disease." in: Glia, Vol. 64, Issue 11, pp. 1841-56, (2018) (PubMed).

Target Details for Huntingtin

Target: Huntingtin (HTT)

Alternative Name: HTT

Background:

Huntingtin is a protein linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons.

HD, HTT and IT15 antibody.

Pathways: PI3K-Akt Signaling, Hormone Transport, Transition Metal Ion Homeostasis, Tube Formation, Protein targeting to Nucleus, Dicarboxylic Acid Transport