Vimentin antibody (C-Term)

Catalog No. ABIN6254183

The Goat Polyclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human, Rat, Mouse, Pig, Cow, Monkey, Chicken, Dog, Cat, Rabbit, Goat, Sheep, Hamster, Horse, Guinea Pig, Avian and Donkey. It has been validated for WB.

Quick Overview for Vimentin antibody (C-Term) (ABIN6254183)

Target: Vimentin (VIM)

Reactivity: Human, Rat, Mouse, Pig, Cow, Monkey, Chicken, Dog, Cat, Rabbit, Goat, Sheep, Hamster, Horse, Guinea Pig, Avian, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Vimentin Antibody

Binding Specificity: C-Term

Purpose: Anti-Vimentin

Sequence: ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE

Specificity: Detects endogenous levels of VIM by Western blot.

Characteristics: Vimentin

Purification: Epitope affinity purified

Immunogen: Recombinant peptide derived from within residues 390 aa to the C-terminus of human VIM produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:5,000, IF:1:100-1:500

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Goat polyclonal antibody to Vimentin. VIM is a member of the intermediate filament family. The cytoskeleton is made by these intermediate filaments, along with actin microfilaments and microtubules. VIM is responsible for integrity of the cytoplasm, stabilization of the cytoskeletal interactions and maintaining cell shape. This protein controls the transport of LDL-derived cholesterol from a lysosome to the site of esterification and is also involved in the immune response. It functions as an organizer of a number of critical proteins involved in migration, attachment and cell signaling.

CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM antibody.

Pathways: Caspase Cascade in Apoptosis