TNF alpha antibody

Catalog No. ABIN6254207

The Goat Polyclonal anti-TNF alpha antibody is suitable to detect TNF alpha in samples from Human, Mouse, Rat, Rabbit, Pig, Dog, Cow, Sheep, Monkey, Cat, Horse, Guinea Pig, Goat, Chicken, Hamster, Avian and Donkey. It has been validated for WB.

Quick Overview for TNF alpha antibody (ABIN6254207)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Dog, Cow, Sheep, Monkey, Cat, Horse, Guinea Pig, Goat, Chicken, Hamster, Avian, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-TNF alpha Antibody

Purpose: anti-TNF alpha

Sequence: MSTESMIRDV ELAEEALPKK TGGPQGSRRC LFLSLFSFLI VAGATTLFCL LHFGVIGPQR EEFPRDLSLI SPLAQAVRSS SRTPSDKPVA HVVANPQAEG QLQWLNRRAN ALLANGVELR DNQLVVPSEG LYLIYSQVLF KGQGCPSTHV LLTHTISRIA VSYQTKVNLL SAIKSPCQRE TPEGAEAKPW YEPIYLGGVF QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL

Specificity: Detects a band of approximately 17 kDa using Raw264.7 cell line by Western blot.

Characteristics: Tumor necrosis factor

Purification: Epitope affinity purified

Immunogen: Purified recombinant human TNF-_ produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF alpha

Background:

Goat polyclonal antibody to TNF alpha. TNF-α is produced by a variety of immune cells including NK cells, B cells, T cells and macrophages. It is multifunctional proinflammatory cytokine that belongs to the tumour necrosis factor (TNF) superfamily. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is involved in the regulation of several processes including lipid metabolism cell differentiation and proliferation, coagulation and apoptosis. It has been implicated in a variety of diseases, including cancer and autoimmune diseases.

APC1, cachectin, tumor necrosis factor ligand superfamily member 2, macrophage-derived TNF, TNF-a, TNF monocyte-derived, tumor necrosis factor-alpha antibody.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome