HIF1A antibody (C-Term)

Catalog No. ABIN6254227

Product Details anti-HIF1A Antibody

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Chicken, Rabbit, Dog, Pig, Monkey, Goat, Horse, Hamster, Guinea Pig, Sheep, Avian, Cat, Donkey

Host: Goat

Clonality: Polyclonal

Conjugate: This HIF1A antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Detects HIF1a in transfected cells and a band of approximately 120 kDa by Western blot in cell lysates.

Characteristics: Hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

Purification: epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 780 aa to the C-terminus of human HIF1a produced in E. coli.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-HIF1A antibody (ABIN6254227)

Ferreira, Rosa Soares, Ramalho, Ribeiro-Rodrigues, Máximo, Zuzarte, Girão, Pereira: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).

Rodrigues, Matafome, Sereno, Almeida, Castelhano, Gamas, Neves, Gonçalves, Carvalho, Arslanagic, Wilcken, Fonseca, Simões, Conde, Castelo-Branco, Seiça: "Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance." in: Scientific reports, Vol. 7, Issue 1, pp. 1698, (2018) (PubMed).

Sacramento, Ribeiro, Rodrigues, Guarino, Diogo, Seiça, Monteiro, Matafome, Conde: "Insulin resistance is associated with tissue-specific regulation of HIF-1α and HIF-2α during mild chronic intermittent hypoxia." in: Respiratory physiology & neurobiology, Vol. 228, pp. 30-8, (2017) (PubMed).

Ferreira, Soares, Ramalho, Pereira, Girao: "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy." in: Scientific reports, Vol. 5, pp. 10210, (2016) (PubMed).

Target Details for HIF1A

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Alternative Name: HIF1a (HIF1A Products)

Pathways: Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, Warburg Effect