Caspase 9 antibody (Cleaved-Asp330)

Catalog No. ABIN6254880

There is 1 publication for this product available. The Rabbit Polyclonal anti-Caspase 9 antibody is suitable to detect Caspase 9 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, IF and ICC.

Quick Overview for Caspase 9 antibody (Cleaved-Asp330) (ABIN6254880)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Caspase 9 Antibody

Binding Specificity: Cleaved-Asp330

Specificity: Cleaved-Caspase 9 (Asp330) Antibody detects endogenous levels of fragment of activated Caspase 9 resulting from cleavage adjacent to Asp330.

Predicted Reactivity: Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Caspase 9 (Cleaved-Asp330).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Caspase 9 antibody (ABIN6254880)

Ding, Weng, Fan, Cao, Lu: "Purkinje Cell Degeneration and Motor Coordination Deficits in a New Mouse Model of Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay." in: Frontiers in molecular neuroscience, Vol. 10, pp. 121, (2017) (PubMed).

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: CASP9

Background:

Description: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).

Gene: CASP9

Molecular Weight: 10 kDa

Gene ID: 842

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity