Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN6254917

Cited in 5+ publications. The Rabbit Polyclonal anti-Smooth Muscle Actin antibody (ABIN6254917) specifically detects Smooth Muscle Actin in WB, IHC, IF, ICC, ELISA and IP. The antibody is reactive with Human, Rat, Mouse and Cow samples.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN6254917)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA, Immunoprecipitation (IP)

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Specificity: Alpha-SMA Antibody detects endogenous levels of total alpha-SMA.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human alpha-SMA, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1000, IHC: 1:50-1:500, IF/ICC: 1:200, IP 1:100, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 15 months

References for anti-Smooth Muscle Actin antibody (ABIN6254917)

Xiaojun, Yan, Hong, Xianghong, Shifeng, Dingjie, Xuemin, Lijuan, Bonan, Zhongqiu, Ruimin, Brann, Fang: "Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica." in: Scientific reports, Vol. 6, pp. 32257, (2018) (PubMed).

Qiu, Wu, Ma, Cao, Huang, Qiu, Peng, Ding: "DL-3-n-Butylphthalide reduces atrial fibrillation susceptibility by inhibiting atrial structural remodeling in rats with heart failure." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 391, Issue 3, pp. 323-334, (2018) (PubMed).

Liu, Pan, Zhao, Liu: "MicroRNA-223 inhibits deposition of the extracellular matrix by airway smooth muscle cells through targeting IGF-1R in the PI3K/Akt pathway." in: American journal of translational research, Vol. 10, Issue 3, pp. 744-752, (2018) (PubMed).

Li, Xiao, Yang, Qin, Gao, Liu, Zhou: "Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway." in: Respiratory research, Vol. 19, Issue 1, pp. 111, (2018) (PubMed).

Zhao, Gao, Xia, Che, Wang, Liu, Sun, Ren, Han, Yang, Lei: "TGF-β1 promotes Staphylococcus aureus adhesion to and invasion into bovine mammary fibroblasts via the ERK pathway." in: Microbial pathogenesis, Vol. 106, pp. 25-29, (2017) (PubMed).

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2

Background:

Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene: ACTA2

Molecular Weight: 45 kDa

Gene ID: 59

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development