alpha-SMA (N-Term) antibody

Catalog No. ABIN6254917

Cited in 5+ publications. The Rabbit Polyclonal anti-alpha-SMA antibody (ABIN6254917) specifically detects alpha-SMA in WB, IHC, IF, IP and ICC. The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for alpha-SMA (N-Term) antibody (ABIN6254917)

Target: alpha-SMA

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details

Binding Specificity: N-Term

Purpose: alpha-SMA Antibody

Specificity: Alpha-SMA Antibody detects endogenous levels of total alpha-SMA.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human alpha-SMA, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC 1:50-1:500, IF/ICC: 1:200, IP 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.Stable for 15 months from date of receipt.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12-18 months

References

Xiaojun, Yan, Hong, Xianghong, Shifeng, Dingjie, Xuemin, Lijuan, Bonan, Zhongqiu, Ruimin, Brann, Fang: "Acetylated α-Tubulin Regulated by N-Acetyl-Seryl-Aspartyl-Lysyl-Proline(Ac-SDKP) Exerts the Anti-fibrotic Effect in Rat Lung Fibrosis Induced by Silica." in: Scientific reports, Vol. 6, pp. 32257, (2018) (PubMed).

Qiu, Wu, Ma, Cao, Huang, Qiu, Peng, Ding: "DL-3-n-Butylphthalide reduces atrial fibrillation susceptibility by inhibiting atrial structural remodeling in rats with heart failure." in: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 391, Issue 3, pp. 323-334, (2018) (PubMed).

Liu, Pan, Zhao, Liu: "MicroRNA-223 inhibits deposition of the extracellular matrix by airway smooth muscle cells through targeting IGF-1R in the PI3K/Akt pathway." in: American journal of translational research, Vol. 10, Issue 3, pp. 744-752, (2018) (PubMed).

Li, Xiao, Yang, Qin, Gao, Liu, Zhou: "Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway." in: Respiratory research, Vol. 19, Issue 1, pp. 111, (2018) (PubMed).

Zhao, Gao, Xia, Che, Wang, Liu, Sun, Ren, Han, Yang, Lei: "TGF-β1 promotes Staphylococcus aureus adhesion to and invasion into bovine mammary fibroblasts via the ERK pathway." in: Microbial pathogenesis, Vol. 106, pp. 25-29, (2017) (PubMed).

Target Details

Target: alpha-SMA

Background:

Description: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Gene: ACTA2

Molecular Weight: 45 kDa

Gene ID: 59

UniProt: P62736