alpha-SMA (AA 84-134) antibody

Catalog No. ABIN8119693

The Rabbit Polyclonal anti-alpha-SMA antibody (ABIN8119693) specifically detects alpha-SMA in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for alpha-SMA (AA 84-134) antibody (ABIN8119693)

Target: alpha-SMA

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: AA 84-134

Purpose: α-SMA Antibody

Specificity: α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human alpha-SMA. AA range: 84-134

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details

Target: alpha-SMA

Background:

Synonyms: ACTA2, ACTSA, ACTVS, GIG46, Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Background: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008],

Gene Name: ACTA2

Protein Name: Actin aortic smooth muscle

Gene ID: 59

UniProt: P62736