alpha-SMA antibody

Catalog No. ABIN8119694

This Rabbit Polyclonal antibody specifically detects alpha-SMA in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for alpha-SMA antibody (ABIN8119694)

Target: alpha-SMA

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Purpose: α-SMA Antibody

Specificity: α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human alpha-SMA.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details

Target: alpha-SMA

Background:

Synonyms: ACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1, ACTA2, ACTSA, ACTVS, GIG46, Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actinACTA1, ACTA, Actin, alpha skeletal muscle, Alpha-actin-1, ACTA2, ACTSA, ACTVS, GIG46, Actin, aortic smooth muscle, Alpha-actin-2, Cell growth-inhibiting gene 46 protein, ACTC1, ACTC, Actin, alpha cardiac muscle 1, Alpha-cardiac actin

Background: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008],

Gene Name: ACTA1/ACTA2/ACTC1

Protein Name: Actin alpha skeletal muscle/Actin aortic smooth muscle/Actin alpha cardiac muscle 1

Gene ID: 59

UniProt: P68133