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ICAM1 antibody (pTyr512)

This Rabbit Polyclonal antibody specifically detects ICAM1 in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.
Catalog No. ABIN6255938

Quick Overview for ICAM1 antibody (pTyr512) (ABIN6255938)

Target

See all ICAM1 Antibodies
ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity

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  • 45
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  • 11
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  • 1
  • 1
Human, Mouse

Host

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  • 22
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Rabbit

Clonality

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Polyclonal

Conjugate

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  • 1
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This ICAM1 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Binding Specificity

    • 22
    • 9
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    pTyr512

    Specificity

    Phospho-ICAM-1 (Tyr512) Antibody detects endogenous levels of ICAM-1 only when phosphorylated at Tyrosine 512

    Cross-Reactivity

    Human, Mouse (Murine)

    Purification

    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

    Immunogen

    A synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyrosine 512

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C.Stable for 12 months from date of receipt

    Expiry Date

    12 months
  • Target

    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

    Alternative Name

    ICAM-1

    Target Type

    Viral Protein

    Background

    Description: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

    Gene: ICAM1

    Molecular Weight

    92kDa

    Gene ID

    3383

    UniProt

    P05362

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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