ICAM1 antibody (pTyr512)

Catalog No. ABIN6255938

This Rabbit Polyclonal antibody specifically detects ICAM1 in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human and Mouse.

Quick Overview for ICAM1 antibody (pTyr512) (ABIN6255938)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ICAM1 Antibody

Binding Specificity: pTyr512

Specificity: Phospho-ICAM-1 (Tyr512) Antibody detects endogenous levels of ICAM-1 only when phosphorylated at Tyrosine 512

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human ICAM-1 around the phosphorylation site of Tyrosine 512

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: ICAM-1

Background:

Description: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.

Gene: ICAM1

Molecular Weight: 92kDa

Gene ID: 3383

UniProt: P05362

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling