Connexin 43/GJA1 antibody (pSer262)

Catalog No. ABIN6256226

This Rabbit Polyclonal antibody specifically detects Connexin 43/GJA1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Connexin 43/GJA1 antibody (pSer262) (ABIN6256226)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: pSer262

Purpose: Phospho-Connexin 43 / GJA1 (Ser262) Antibody

Specificity: Phospho-Cx43(Ser262) Antibody detects endogenous levels of Cx43.

Predicted Reactivity: Rat,Pig,Bovine,Sheep,Rabbit

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Connexin 43 / GJA1 around the phosphorylation site of Ser262.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43 / GJA1

Background:

Description: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

Gene: GJA1

Molecular Weight: 42, 44, 46kDa

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization