MAPT antibody (pThr231)

Catalog No. ABIN6256514

This Rabbit Polyclonal antibody specifically detects MAPT in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for MAPT antibody (pThr231) (ABIN6256514)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MAPT Antibody

Binding Specificity: pThr231

Specificity: Phospho-Tau (Thr231) Antibody detects endogenous levels of Tau only when phosphorylated at Thr548, which site historically referenced as Thr231.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog,Chicken

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Tau around the phosphorylation site of Thr231.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT

Background:

Description: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Gene: MAPT

Molecular Weight: 78kDa

Gene ID: 4137

UniProt: P10636

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size