Choline Acetyltransferase antibody (C-Term)

Catalog No. ABIN6256821

The Rabbit Polyclonal anti-Choline Acetyltransferase antibody has been validated for WB, IHC and ELISA. It is suitable to detect Choline Acetyltransferase in samples from Human, Mouse and Rat.

Quick Overview for Choline Acetyltransferase antibody (C-Term) (ABIN6256821)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Choline Acetyltransferase Antibody

Binding Specificity: C-Term

Specificity: Choline Acetyltransferase Antibody detects endogenous levels of total Choline Acetyltransferase.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Choline Acetyltransferase, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: CHAT

Background:

Description: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Gene: CHAT

Molecular Weight: 82kDa

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development