NDFIP2 antibody (Internal Region)

Catalog No. ABIN6257381

This Rabbit Polyclonal antibody specifically detects NDFIP2 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NDFIP2 antibody (Internal Region) (ABIN6257381)

Target: NDFIP2 (Nedd4 Family Interacting Protein 2 (NDFIP2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDFIP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NDFIP2 Antibody

Binding Specificity: Internal Region

Purpose: NDFIP2 Antibody

Specificity: NDFIP2 Antibody detects endogenous levels of total NDFIP2.

Predicted Reactivity: Pig,Bovine,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NDFIP2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NDFIP2

Target: NDFIP2 (Nedd4 Family Interacting Protein 2 (NDFIP2))

Alternative Name: NDFIP2

Background:

Description: Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).

Gene: NDFIP2

Molecular Weight: 36 kDa

Gene ID: 54602

UniProt: Q9NV92

Pathways: Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome