CPSF6 antibody (Internal Region)

Catalog No. ABIN6261008

Product Details anti-CPSF6 Antibody

Target: CPSF6 (Cleavage and Polyadenylation Specific Factor 6, 68kDa (CPSF6))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: CPSF6 Antibody detects endogenous levels of total CPSF6.

Predicted Reactivity: Bovine,Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CPSF6, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CPSF6

Target: CPSF6 (Cleavage and Polyadenylation Specific Factor 6, 68kDa (CPSF6))

Alternative Name: CPSF6 (CPSF6 Products)

Synonyms: CPSF6 antibody, wu:fa22f12 antibody, zgc:85819 antibody, 4733401N12Rik antibody, AI256641 antibody, CFIM antibody, CFIM68 antibody, HPBRII-4 antibody, HPBRII-7 antibody, cleavage and polyadenylation specific factor 6 antibody, cleavage and polyadenylation specific factor 6 S homeolog antibody, CPSF6 antibody, cpsf6.S antibody, cpsf6 antibody, Cpsf6 antibody

Background:

Description: Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921, PubMed:8626397, PubMed:14690600, PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921, PubMed:8626397, PubMed:14690600). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700, PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905, PubMed:29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery (PubMed:15169763, PubMed:29276085, PubMed:21295486). Plays a role in mRNA export (PubMed:19864460).

Gene: CPSF6

Molecular Weight: 59 kDa

Gene ID: 11052

UniProt: Q16630