DCLRE1B antibody (Internal Region)

Catalog No. ABIN6261237

Product Details anti-DCLRE1B Antibody

Target: DCLRE1B (DNA Cross-Link Repair 1B (DCLRE1B))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLRE1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: DCLRE1B Antibody detects endogenous levels of total DCLRE1B.

Predicted Reactivity: Pig,Bovine,Horse,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DCLRE1B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DCLRE1B

Target: DCLRE1B (DNA Cross-Link Repair 1B (DCLRE1B))

Alternative Name: DCLRE1B (DCLRE1B Products)

Synonyms: APOLLO antibody, SNM1B antibody, SNMIB antibody, im:6894074 antibody, zgc:154089 antibody, AI452214 antibody, Apollo antibody, mSNM1B antibody, DNA cross-link repair 1B antibody, DCLRE1B antibody, Dclre1b antibody, dclre1b antibody

Background:

Description: 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint.

Gene: DCLRE1B

Molecular Weight: 60 kDa

Gene ID: 64858

UniProt: Q9H816