DIS3 antibody (Internal Region)

Catalog No. ABIN6261304

Product Details anti-DIS3 Antibody

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: DIS3 Antibody detects endogenous levels of total DIS3.

Predicted Reactivity: Pig,Zebrafish,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DIS3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DIS3

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Alternative Name: DIS3 (DIS3 Products)

Synonyms: EXOSC11 antibody, KIAA1008 antibody, RP11-342J4.3 antibody, RRP44 antibody, bA555G22.1 antibody, dis3p antibody, DIS3 antibody, 2810028N01Rik antibody, RGD1304646 antibody, wu:fb08g12 antibody, dis3 antibody, DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease antibody, DIS3 exosome endoribonuclease and 3'-5' exoribonuclease antibody, DIS3 antibody, Dis3 antibody, dis3 antibody, dis3.L antibody

Background:

Description: Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Gene: DIS3

Molecular Weight: 110 kDa

Gene ID: 22894

UniProt: Q9Y2L1