GFM1 antibody (C-Term)

Catalog No. ABIN6261942

The Rabbit Polyclonal anti-GFM1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect GFM1 in samples from Human.

Quick Overview for GFM1 antibody (C-Term) (ABIN6261942)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GFM1 Antibody

Binding Specificity: C-Term

Specificity: GFM1 Antibody detects endogenous levels of total GFM1.

Predicted Reactivity: Pig,Zebrafish,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human GFM1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Alternative Name: GFM1

Background:

Description: Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Does not mediate the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis.

Gene: GFM1

Molecular Weight: 83 kDa

Gene ID: 85476

UniProt: Q96RP9