MED15 antibody (C-Term)

Catalog No. ABIN6263190

The Rabbit Polyclonal anti-MED15 antibody has been validated for WB, ELISA and IHC. It is suitable to detect MED15 in samples from Human, Mouse and Rat.

Quick Overview for MED15 antibody (C-Term) (ABIN6263190)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MED15 Antibody

Binding Specificity: C-Term

Specificity: MED15 Antibody detects endogenous levels of total MED15.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MED15, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Background:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.

Gene: MED15

Molecular Weight: 87kDa

Gene ID: 51586

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha