MED15 antibody (AA 510-590)

Catalog No. ABIN7229622

The Rabbit Polyclonal anti-MED15 antibody is suitable to detect MED15 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for MED15 antibody (AA 510-590) (ABIN7229622)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MED15 Antibody

Binding Specificity: AA 510-590

Purpose: Rabbit Anti-MED15 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MED15 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MED15 protein at AA range: 510-590

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Alternative Name: MED15

Background: Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, TIG-1, Trinucleotide repeat-containing gene 7 proteinThe protein encoded by MED15 (Mediator Complex Subunit 15) is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. MED15 contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants.

Molecular Weight: 86kD

Gene ID: 51586

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha