LEPREL2 antibody (Internal Region)

Catalog No. ABIN6263960

Product Details anti-LEPREL2 Antibody

Target: LEPREL2 (Leprecan Like Protein 2 (LEPREL2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LEPREL2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: P3H3 Antibody detects endogenous levels of total P3H3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human P3H3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LEPREL2

Target: LEPREL2 (Leprecan Like Protein 2 (LEPREL2))

Alternative Name: P3H3 (LEPREL2 Products)

Synonyms: GRCB antibody, HSU47926 antibody, P3H3 antibody, BC016431 antibody, Grcb antibody, P3h3 antibody, prolyl 3-hydroxylase 3 antibody, P3H3 antibody, P3h3 antibody

Background:

Description: Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea. Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly. Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases.

Gene: P3H3

Molecular Weight: 82 kDa

Gene ID: 10536

UniProt: Q8IVL6