Prosaposin antibody (Internal Region)

Catalog No. ABIN6264456

The Rabbit Polyclonal anti-Prosaposin antibody is suitable to detect Prosaposin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA, ICC and IF.

Quick Overview for Prosaposin antibody (Internal Region) (ABIN6264456)

Target: Prosaposin (PSAP)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Prosaposin Antibody

Binding Specificity: Internal Region

Specificity: PSAP Antibody detects endogenous levels of total PSAP.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PSAP, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: PSAP

Background:

Description: Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.

Gene: PSAP

Molecular Weight: 58kDa

Gene ID: 5660

UniProt: P07602

Pathways: Positive Regulation of Endopeptidase Activity