PSMB9 antibody (Internal Region)

Catalog No. ABIN6264478

Product Details anti-PSMB9 Antibody

Target: PSMB9 (Proteasome (Prosome, Macropain) Subunit, beta Type, 9 (Large Multifunctional Peptidase 2) (PSMB9))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMB9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: Proteasome 20S LMP2 Antibody detects endogenous levels of total Proteasome 20S LMP2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Proteasome 20S LMP2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-PSMB9 antibody (ABIN6264478)

Liu, Wu, Zhu, Li, Liu: "IRG1 increases MHC class I level in macrophages through STAT-TAP1 axis depending on NADPH oxidase mediated reactive oxygen species." in: International immunopharmacology, Vol. 48, pp. 76-83, (2018) (PubMed).

Target Details for PSMB9

Target: PSMB9 (Proteasome (Prosome, Macropain) Subunit, beta Type, 9 (Large Multifunctional Peptidase 2) (PSMB9))

Alternative Name: PSMB9 (PSMB9 Products)

Synonyms: LMP2 antibody, ring12 antibody, MHC-linked antibody, psmb9b antibody, lmp2 antibody, lmp2b antibody, psmb9 antibody, Lmp-2 antibody, Lmp2 antibody, PSMB6i antibody, RING12 antibody, beta1i antibody, psmb9-A antibody, macropain antibody, prosome antibody, wu:fa66f04 antibody, wu:fj22d05 antibody, zgc:91842 antibody, psmb9a antibody, proteasome subunit beta 9 antibody, LMP2 antibody, proteasome subunit beta type-9b antibody, proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2) antibody, proteasome subunit beta 9 S homeolog antibody, proteasome subunit beta 9a antibody, proteasome (prosome, macropain) subunit, beta type, 9a antibody, psmb9 antibody, PSMB9 antibody, psmb9-b antibody, Psmb9 antibody, psmb9.S antibody, psmb9a antibody

Background:

Description: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic  pH . The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues.

Gene: PSMB9

Molecular Weight: 23kDa

Gene ID: 5698

UniProt: P28065

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA