p130 antibody (C-Term)

Catalog No. ABIN6264666

The Rabbit Polyclonal anti-p130 antibody has been validated for WB and ELISA. It is suitable to detect p130 in samples from Human and Rat.

Quick Overview for p130 antibody (C-Term) (ABIN6264666)

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p130 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-p130 Antibody

Binding Specificity: C-Term

Specificity: RBL2 Antibody detects endogenous levels of total RBL2.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RBL2, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for p130

Target: p130 (RBL2) (Retinoblastoma-Like 2 (p130) (RBL2))

Alternative Name: RBL2

Background:

Description: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.

Gene: RBL2

Molecular Weight: 128kDa

Gene ID: 5934

UniProt: Q08999

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases