Smad2/3 antibody (Thr8)

Catalog No. ABIN6280124

Product Details anti-Smad2/3 Antibody

Target: Smad2/3 (SMAD2/3) (Smad2/3 Transcription Factor (SMAD2/3))

Binding Specificity: AA 1-80, Thr8

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Smad2/3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Smad2/3 is a protein encoded by the SMAD2 gene which is approximately 52,3 kDa. Smad2/3 is localised to the cytoplasm and nucleus. It is involved in TGF-beta receptor signalling which activate SMADs, Th17 cell differentiation and human embryonic stem cell pluripotency. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signalling pathways. This protein mediates the signal of the TGF-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation protein. Smad2/3 is expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. Mutations in the SMAD2 gene may result in Ureteral disease. STJ95697 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Smad2/3 protein.

Specificity: Smad2/3 Polyclonal Antibody detects endogenous levels of Smad2/3 protein.

Characteristics: Rabbit polyclonal to Smad2/3.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Smad2/3 around the non-phosphorylation site of T8.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000

Comment:

Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Smad2/3

Target: Smad2/3 (SMAD2/3) (Smad2/3 Transcription Factor (SMAD2/3))

Alternative Name: Smad2/3 (SMAD2/3 Products)

Molecular Weight: 48/60 kDa

Gene ID: 4087

UniProt: Q15796