RIP2 antibody (pSer176)

Catalog No. ABIN6281084

Product Details anti-RIP2 Antibody

Target: RIP2 (ROP Interactive Partner 2 (RIP2))

Binding Specificity: AA 120-200, pSer176

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: RIP2 is a protein encoded by the RIPK2 gene which is approximately 61,2 kDa. RIP2 is localised to the cytoplasm. It is involved in activated TLR4 signalling, p75 NTR receptor-mediated signalling, toll-like receptor signalling pathways and cytokine signalling in immune system. It is a serine/threonine/tyrosine kinase that contains a C-terminal caspase activation and recruitment domain and plays an essential role in modulation of innate and adaptive immune responses. RIP2 is expressed in the heart, brain, placenta, lung, peripheral blood leukocytes, spleen and kidney. Mutations in the RIPK2 gene may result in x-linked lymphoproliferative syndrome and human granulocytic anaplasmosis. STJ90957 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous RIP2 protein which only binds about S176 when S176 is phosphorylated.

Specificity: Phospho-RIP2 (S176) Polyclonal Antibody detects endogenous levels of RIP2 protein only when phosphorylated at S176.

Characteristics: Rabbit polyclonal to Phospho-RIP2 (S176).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human RIP2 around the phosphorylation site of S176.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Comment:

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for RIP2

Target: RIP2 (ROP Interactive Partner 2 (RIP2))

Alternative Name: RIP2 (RIP2 Products)

Molecular Weight: 70 kDa

Gene ID: 8767

UniProt: O43353

Pathways: TCR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades