PKC antibody (AA 600-680)

Catalog No. ABIN6284619

Product Details anti-PKC Antibody

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Binding Specificity: AA 600-680

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: PKC is a protein encoded by the PRKCA gene which is approximately 76,7 kDa. PKC is localised to the cytoplasm and cell membrane. It is involved in RET signalling, DAG and IP3 signalling, the IL-2 Pathway, fMLP pathway and CDK-mediated phosphorylation and removal of Cdc6. It is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. It plays a role in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. PKC is expressed in the liver, blood, nervous system, skin and pancreas. Mutations in the PRKCA gene may result in glioma and myotonic dystrophy. STJ95117 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of PKC protein.

Specificity: PKC Polyclonal Antibody detects endogenous levels of PKC protein.

Characteristics: Rabbit polyclonal to PKC.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PKC around the non-phosphorylation site of T497.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for PKC

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Alternative Name: PKC (PKC pan Products)

Synonyms: PKC antibody, PKCI antibody, Prkc antibody, Prkcc antibody, RATPKCI antibody, protein kinase C, gamma antibody, Prkcg antibody

Molecular Weight: 85 kDa

Gene ID: 5578

UniProt: P17252

Pathways: Positive Regulation of Response to DNA Damage Stimulus