EpCAM antibody (AA 20-60)

Catalog No. ABIN6294377

The Rabbit Polyclonal anti-EpCAM antibody is suitable to detect EpCAM in samples from Human. It has been validated for WB, FACS, IHC and IF.

Quick Overview for EpCAM antibody (AA 20-60) (ABIN6294377)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EpCAM Antibody

Binding Specificity: AA 20-60

Purpose: Rabbit anti-Human/rat Recombinant EpCAM Antibody [Sodium Azide Free]

Specificity: Cell surface, cytoplasmic

Purification: A portion of amino acids 20-60 was used as the immunogen for the recombinant EpCAM antibody.

Immunogen: A portion of amino acids 20-60 was used as the immunogen for the recombinant EpCAM antibody.

Application Details

Application Notes: Western blot: 0.5-1 μg/mL
IF: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Epithelial cell adhesion molecule

Background:

Target Description: Recognizes a 40-43 kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. It is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Gene Symbol: EPCAM///GA733-2///M1S2///M4S1///MIC18///TACSTD1///TROP1

Gene ID: 4072

UniProt: P16422