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EpCAM antibody (AA 27-59)

This Mouse Monoclonal antibody specifically detects EpCAM in WB, FACS, IHC and IF. It exhibits reactivity toward Human.
Catalog No. ABIN6294439
$739.45
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 6 to 9 Business Days

Quick Overview for EpCAM antibody (AA 27-59) (ABIN6294439)

Target

See all EpCAM (EPCAM) Antibodies
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This EpCAM antibody is un-conjugated

Application

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Binding Specificity

    • 86
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    • 35
    • 27
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    AA 27-59

    Purpose

    Mouse anti-Human EpCAM Antibody [Sodium Azide Free]

    Specificity

    Cell surface

    Purification

    Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.

    Immunogen

    Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.
  • Application Notes

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL
    Western blot: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    Restrictions

    For Research Use only
  • Buffer

    In 1X PBS, BSA free, sodium azide free

    Preservative

    Azide free

    Storage

    4 °C,-20 °C

    Storage Comment

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • Target

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    Alternative Name

    Epithelial cell adhesion molecule

    Background

    Target Description: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

    Gene Symbol: EPCAM///GA733-2///M1S2///M4S1///MIC18///TACSTD1///TROP1

    Gene ID

    4072

    UniProt

    P16422
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