EpCAM antibody (AA 27-59)
Quick Overview for EpCAM antibody (AA 27-59) (ABIN6294439)
Target
See all EpCAM (EPCAM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 27-59
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Purpose
- Mouse anti-Human EpCAM Antibody [Sodium Azide Free]
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Specificity
- Cell surface
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Purification
- Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.
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Immunogen
- Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for this EpCAM antibody. The epitope has been localized to amino acids 27-59.
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Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
Restrictions
- For Research Use only
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Buffer
- In 1X PBS, BSA free, sodium azide free
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Preservative
- Azide free
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Storage
- 4 °C,-20 °C
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Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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Alternative Name
- Epithelial cell adhesion molecule
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Background
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Target Description: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Gene Symbol: EPCAM///GA733-2///M1S2///M4S1///MIC18///TACSTD1///TROP1
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Gene ID
- 4072
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UniProt
- P16422
Target
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