GZMB antibody (AA 73-187)

Catalog No. ABIN6294582

This anti-GZMB antibody is a Rabbit Polyclonal antibody detecting GZMB in WB, IHC, FACS and IF. Suitable for Human.

Quick Overview for GZMB antibody (AA 73-187) (ABIN6294582)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-GZMB Antibody

Binding Specificity: AA 73-187

Purpose: Rabbit anti-Human Granzyme B Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.

Immunogen: A recombinant fragment (amino acids 73-187) from the human protein was used as the immunogen for the Granzyme B antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B

Background:

Target Description: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'non-self' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Gene Symbol: GZMB///CGL1///CSPB///CTLA1///GRB

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis