KRT8 antibody

Catalog No. ABIN6295954

This Mouse Monoclonal antibody specifically detects KRT8 in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Rat and Zebrafish (Danio rerio).

Quick Overview for KRT8 antibody (ABIN6295954)

Target: KRT8 (Keratin 8 (KRT8))

Reactivity: Human, Rat, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-KRT8 Antibody

Purpose: Mouse anti-Human/Rat/Zebrafish Cytokeratin 8 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A cytoskeleton preparation containing Cytokeratin 8 was used as the immunogen for this antibody.

Immunogen: A cytoskeleton preparation containing Cytokeratin 8 was used as the immunogen for this antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for KRT8

Target: KRT8 (Keratin 8 (KRT8))

Alternative Name: Keratin, type II cytoskeletal 8

Background:

Target Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. antibody to Cytokeratin 8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against Cytokeratin 8, 18 and 19). Reportedly, Cytokeratin 8 antibody is useful for the differentiation of lobular ( ""ring-like, perinuclear "") from ductal ( ""peripheral-predominant "") carcinoma of the breast.

Gene Symbol: KRT8///CYK8

Gene ID: 3856

UniProt: P05787