CD34 antibody

Catalog No. ABIN6295964

The Mouse Monoclonal anti-CD34 antibody is suitable to detect CD34 in samples from Human, Cynomolgus and Rhesus Monkey. It has been validated for FACS, IHC and IF.

Quick Overview for CD34 antibody (ABIN6295964)

Target: CD34

Reactivity: Human, Cynomolgus, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD34 Antibody

Purpose: Mouse anti-Human/Cynomolgus Monkey/Rhesus Monkey CD34 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Immunogen: A detergent solubilized vesicular suspension prepared from human term placenta was used as the immunogen for this CD34 antibody.

Application Details

Application Notes: FACS: use antibody at 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD34

Target: CD34

Alternative Name: Hematopoietic progenitor cell antigen CD34

Background:

Target Description: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi 's sarcoma, but with a lower specificity.

Gene Symbol: CD34

Gene ID: 947

UniProt: P28906