Vimentin antibody
Quick Overview for Vimentin antibody (ABIN6296018)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Purpose
- Mouse anti-Human/Cat/Cow/Pig/Rat/Rabbit/Mouse/Sheep Vimentin Antibody [Sodium Azide Free]
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Specificity
- Cytoplasmic
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Purification
- A human thymic nuclear extract was used as the immunogen for the Vimentin antibody.
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Immunogen
- A human thymic nuclear extract was used as the immunogen for the Vimentin antibody.
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Application Notes
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2) -
Restrictions
- For Research Use only
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Buffer
- In 1X PBS, BSA free, sodium azide free
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Preservative
- Azide free
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Storage
- 4 °C,-20 °C
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Storage Comment
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Vimentin (VIM)
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Alternative Name
- Vimentin
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Background
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Target Description: This mAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias.
Gene Symbol: VIM
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Gene ID
- 7431
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Target
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