GFM1 antibody

Catalog No. ABIN6296110

This Mouse Monoclonal antibody specifically detects GFM1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for GFM1 antibody (ABIN6296110)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-GFM1 Antibody

Purpose: Mouse anti-Human Mitochondrial Antibody [Sodium Azide Free]

Specificity: Cytoplasmic (Mitochondria)

Purification: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.

Immunogen: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.

Application Details

Application Notes: ELISA: order Ab without BSA
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Alternative Name: Mitochondrial

Background:

Target Description: This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.