BAX antibody

Catalog No. ABIN6296161

This Mouse Monoclonal antibody specifically detects BAX in WB, IHC, ELISA, IF, FACS and Coat. It exhibits reactivity toward Human and Monkey.

Quick Overview for BAX antibody (ABIN6296161)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Product Details anti-BAX Antibody

Purpose: Mouse anti-human/monkey Bax Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.

Immunogen: Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.

Application Details

Application Notes: ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Apoptosis regulator BAX

Background:

Target Description: This antibody recognizes a protein of 21 kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.

Gene Symbol: BAX///BCL2L4

Gene ID: 581

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response