Cytokeratin 7 antibody

Catalog No. ABIN6296310

The Rabbit Polyclonal anti-Cytokeratin 7 antibody (ABIN6296310) specifically detects Cytokeratin 7 in IHC, IF and FACS. The antibody is reactive with Human samples.

Quick Overview for Cytokeratin 7 antibody (ABIN6296310)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Cytokeratin 7 Antibody

Purpose: Rabbit anti-Human Recombinant Cytokeratin 7 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Full-length human CK7 protein was used as the immunogen for the recombinant Cytokeratin 7 antibody.

Immunogen: Full-length human CK7 protein was used as the immunogen for the recombinant Cytokeratin 7 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10e6 cells
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Keratin, type II cytoskeletal 7

Background:

Target Description: Cytokeratin-7 (CK7) is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK7 is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. It is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain CK7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the protein is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively. It is commonly used together with CK20 when making such diagnoses. [Wiki]

Gene Symbol: KRT7///SCL

Gene ID: 3855

UniProt: P08729